Spectrophotometry

Spectrophotometry is a technique that determines how materials interact with light as a function of wavelength and other attributes of both the material and the light. In contrast to spectrofluorometry, the dispersion performed by the monochromator or interferometer takes place either between the source and the sample or between the sample and the detector, not in both locations. We describe each of these properties and the range of these properties that ODA can address with its four major spectrophotometers.

Wavelength Range
Instrument Nanometer - nm Range Micron - µm Range Wavenumber - 1/cm Range
Agilent Cary 7000
Dispersive UV-Vis-NIR
5° - 80° Angles of Incidence & Exitence
350 2,500 0.35 2.5 28,572 4,000
Agilent Cary 5000
Dispersive UV-Vis-NIR
Normal/Near Normal AOI
200 3,000 0.20 3.0 50,000 3,030
Agilent Cary 500E
Dispersive UV-Vis-NIR
Integrating Sphere
250 2,500 0.25 2.5 40,000 4,000
Perkin-Elmer 983G
Dispersive IR
Normal/Near Normal AOI
2,000 55,600 2.0 55.6 5,000 180
Nicolet 560 FT-IR
Fourier Transform IR
Integrating Sphere
2,000 25,000 2.0 25.0 5,000 400

Optical Arrangement
Instrument Layout Sources Sample Compartment Detectors
Agilent Cary 7000
Dispersive UV-Vis-NIR
Sources - doublemonochromator - sample - detectors Tungsten-halogen/deuterium Double-beam, focus in center Si/InGaAs
Agilent Cary 5000
Dispersive UV-Vis-NIR
Sources - doublemonochromator - sample - detectors Tungsten-halogen/deuterium Double-beam, focus in center PMT, cooled PbS
Agilent Cary 500E
Dispersive UV-Vis-NIR
Sources - doublemonochromator-sample -detectors Tungsten-halogen/deuterium Double-beam, focus in center PMT, cooled PbS
Perkin-Elmer 983G
Dispersive IR
Source-sample-monochromator - detector Globar™ Double-beam converging Thermocouple
Nicolet 560
Fourier Transform IR
Sources- interfero-meter - samples- detector Globar™/tungsten-halogen Single-beam-collimated DTGS

Orientation
Transmittance Angle Reflectance Angle
Incident Exitent Incident Exitent
Normal Direct [Specular] Absolute Near-Normal Specular Absolute
Variable Angle Direct [Specular] Absolute 0 - 85° 0 - 85° Variable Angle Specular Absolute 5° - 85° 5° - 85°
Normal Total Absolute 2π sr Forward Forward Near Normal Total Referenced 2π sr Backward
Variable Angle Total Absolute 0° - 75° 2π sr Forward Variable Angle Total Referenced 15° - 75° 2π sr Backward

Polarization

The incident beams from all four instruments are somewhat polarized due to the presence of gratings and non-normal reflections in the optical train. The parallel - p - and perpendicular - s - polarizations can be specified and isolated with polarizers over the 0.35 < λ < 2500 nm spectral range of the two Agilent SPMs.

ODA does not own IR polarizers for λ > 3000 nm, so polarization cannot be controlled in this wavelength range.

2015, Optical Data Associates, LLC